BRAFV600E mutation in sporadic and neurofibromatosis type 1-related malignant peripheral nerve sheath tumors.

Malignant peripheral nerve sheath tumors (MPNSTs) are rare, but deadly, soft tissue sarcomas that occur more commonly and at a younger age in individuals affected with the neurofibromatosis type 1 (NF1) inherited cancer predisposition syndrome. Currently, the only curative treatment for MPNSTs is surgery, and treatment of advanced disease is limited to cytotoxic chemotherapy with generally little effect on overall survival. Moreover, there is a pressing need for targeted therapeutic options for MPNSTs arising in individuals with NF1 and in the general population. Mutations in the NF1 gene have been reported in both NF1-related and sporadic MPNSTs, such that loss of NF1 protein (neurofibromin) function leads to increased Ras signaling. – 4 Given that the BRAF kinase molecule is also a known downstream Ras effector and that BRAF mutations are found in numerous nervous system cancers, we hypothesized that BRAF mutations may identify a molecularly-distinct subset of MPNST. To explore this hypothesis, we employed immunohistochemistry using a BRAF mutation-specific antibody on formalinfixed/paraffin-embedded samples representing 62 MPNSTs and reviewed the associated clinical data to determine NF1 status, overall survival, and time to metastasis. Representative images are shown in Fig. 1 (left panels). Confirmatory BRAF sequencing was not performed on these tumors, based on our previous experience revealing complete concordance between sequencing and immunohistochemistry results in ganglioglioma as well as in other laboratories using different tumor types. First, we found that individuals with NF1 developed MPNSTs 10–15 years earlier than their sporadic counterparts (P, .003; Fig. 1; right panel), which was similar to previous reports. – 12 Second, BRAF mutation was observed in 20% (5/25) of sporadic MPNSTs. In these BRAF-positive MPNSTs, 90%–100% of the tumor cells were BRAF-immunoreactive, suggesting that this mutation could be a primary driver of malignancy and not merely a mutation found in a subset of tumor cells. The prevalence of BRAF mutation in our series of 62 MPNSTs was slightly higher than previously reported in a smaller series of tumors (1 of 13 sporadic MPNSTs) and was likewise higher than observed in sporadic glioblastoma. In this regard, analysis of available The Cancer Genome Atlas (TCGA) data using the C-BIO website application (http://cbio.mskcc.org) revealed that BRAF mutations predominated in thyroid cancer (182/323 tumors; 56%) and melanoma (81/228 tumors; 36%) but were far less common in all other cancers examined (,9%). Interestingly, melanocytes and Schwann cells have neural crest origins, suggesting a susceptible cell of origin for BRAF mutation in the genesis of these cancers. Third,BRAF mutationwasnotobserved inbenignneurofibromas (0/11 tumors), implicating BRAF mutation in malignant progression rather than in neurofibroma tumorigenesis. Fourth, only one NF1-associated MPNST harbored a BRAF mutation (1/37; 2.7%). There was a statistically significant difference in the percentage of BRAF mutations in NF1-associated MPNSTs relative to their sporadic counterparts (P 1⁄4 .035). While these molecular events might be considered mutually exclusive, BRAF molecular alterations have also been reported in another NF1-associated nervous system tumor (pilocytic astrocytoma). Fifth, BRAF mutationdoesnotappear toconferanystatisticallysignificant differences in overall patient survival or time to metastasis when patients were censored by either date of death or date of last follow-up. This may reflect the small sample size, the rarity of these tumors, and the fact that nearly one-third of the patients were lost to follow-up (and assumed to be living as they could not be found in the Social Security Death Index). While the prognostic value has yet to be established, this study identifies BRAF mutation as a significant molecular alteration in MPNST, potentially offering another therapeutic target for a deadly cancer with no currently efficacious treatment options.